Product Overview

Clinically relevant drug-drug interactions (DDI) are a serious concern for any new drug development project.

To better support capturing the multiple mechanisms of DDI potential using cryopreserved primary human hepatocytes, we now offer Interaction Qualified Cryopreserved Human Hepatocytes (Catalog HUCPI) which are characterized for 3 major mechanisms of DDI:

• Transporter activity
• Enzyme activity
• Enzyme gene induction potential. 

Benefits

• Use the same donor for basal clearance, transport, and induction studies
• Actual rates of transport reported rather than relative rates for less ambiguity
• Large lots mean fewer rounds of testing so you can focus on results
• Prequalified in Lonza media for better reproducibility
• Pre-tested for long-term culture, spheroid formation, and 96-well compatibility

Features:

• Confluent monolayer with cobblestone morphology, distinct nuclei, and tight junctions for 5 days or more
• Inducibility of enzyme activity for CYP3A4, CYP2B6, and CYP1A2
• Inducibility of mRNA for CYP3A4, CYP2B6, CYP1A2, and CYP2C8 genes
• Actual rate of uptake or efflux for OATP1B1/3, OCT1/2, NTCP, and BSEP** transporters
• Differences in passive vs. active uptake for OATP1B1/3, OCT1/2, and NTCP transporters.
• Basal metabolism for 8 CYPs, SULT, UGT, and aldehyde oxidase
• Plated low-turnover clearance for CYP2C9, CYP2D6, and CYP3A4
• Complementary thawing, plating, and maintenance medium available
• > 5 million viable cells/vial
• Characterized for long-term culture potential (> 7 days)
• Characterized for spheroid formation potential

Our qualification methods for cryopreserved human hepatocytes are modelled after recommendations from the FDA publication In Vitro Metabolism and Transporter Mediated Drug-Drug Interaction Studies Guidance for Industry (2017, http://www.fda.gov/Drugs/GuidanceComplianceRegulatoryInformation/Guidances/default.htm) .

Applications

• Drug-Drug interaction studies
• Low clearance metabolism studies
• Drug uptake and efflux
• Disease modeling
• Xenotransplantation
• Short term cellular toxicity studies
• 3D cell culture

Content
Cryopreserved ampule of HUCPI containing >5 million viable cells

Recommended Thawing media

Lonza Human Cryopreserved Hepatocyte Thawing Medium

Recommended Plating Media

Lonza Hepatocyte Plating Medium

Recommended Maintenance Media

Lonza HCM™ Hepatocyte Culture Medium BulletKit™

Catalog #: HUCPI

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Technical information & instructions for enzyme activity assessment

Technical information and instructions for culturing cryopreserved primary hepatocytes in suspension or plated formats

Reference guidefor human and animal cells from normal to diseased, fresh and cryopreserved