Unique RT Primer :
Conformational restricted miRNA specific RT primer efficiently hybridizes to mature but not precursor form of target miRNA.
Specific Real-Time PCR Primers :
miRNA specific forward and reverse real-time PCR primers confer further specificity and enable robust amplification of amplicon.
Tailored RT-qPCR Reagents :
Optimized RT and qPCR master mixes enhance signal to noise ratio.
Optimized RT-qPCR primers and reagents to drive efficient target amplification from limiting amounts (≥1pg) of input RNA sample.
No universal primers. Every assay utilizes three miRNA specific primers to discriminate single nucleotide differences.
RNA to Ct in less than 2 hours for faster turnaround and improved throughput.
Assays optimized by MIRXES’ proprietary algorithm and wet-lab validated with synthetic miRNA templates and RNA from biological samples.
Complete kit to minimize set-up time. Compatible with all major qPCR instruments.