For the rapid purification of total RNA - including microRNA - without the use of phenol

• Extract high quality & quantity total RNA including miRNA

• No phenol step required; isolate all RNA in one fraction

• Bind & elute all RNA irrespective of size or GC content, without bias

• Very sensitive & linear down to a few cells without the need for carrier RNA

• Isolate from a wide variety of specimens

• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing.

• Available in a variety of formats to suit your needs

• Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Total RNA Purification Kits

These kits are suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A₂₆₀/A₂₈₀ suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. These kits purify all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Total RNA Purification 96-Well Kit (High Throughput and High Throughput Deep Well)

This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.

Kit Specifications -- Spin Column

Kit Specifications -- High Throughput

* average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Catalog #: 17200 -- Spin Column, 50 preps

Catalog #: 37500 -- Spin Column, 100 preps

Catalog #: 24300 -- High Throughput, 2 x 96-well plates

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Total RNA Purification Kit (Spin Column) - Protocol (50 prep)

Total RNA Purification Kit (Spin Column) - Protocol (100 prep)

Total RNA Purification 96-Well Kit (High Throughput) - Protocol (96-well) - (2 x 96-well plates)

Supplementary Protocols

Supplementary Protocol - Total RNA Isolation From Cerebrospinal Fluid Csf

Total RNA Purification Kit - Supplementary Protocol for Exosomal RNA Purification from Exosomes Already Purified

Supplementary Protocol for Total RNA Isolation from Staphylococcus aureus-Related Bacteria and Hard-to-Lyse Bacterial Species

Isolation of RNA from Norgen’s Swab Collection and Total Nucleic Acid Preservation System

Supplementary Protocol - Norgen's Saliva RNA Collection and Preservation Devices

Supplementary Protocol for Isolating Viral RNA from Swab Samples

Application Notes

Isolation and Detection of RNA from as Little as a Single Animal Cell Using Norgen’s Total RNA Purification Kit

Isolation of True Total RNA using the Tempus™ Blood RNA Tube and Norgen’s Total RNA Purification Kit

Effect of RNA Isolation Method on microRNA Quantity and Quality in Plasma: A Comparative Study

Revised Guidelines for RNA Quality Assessment for Diverse Biological Sample Input

Effect of microRNA GC Content On RNA Purification Efficiency

Total RNA/DNA Purification and Detection from Blood Preserved on a Neoteryx™ Mitra Microsampling Device

Effect of microRNA GC Content on RNA Purification Efficiency

Safety Data Sheets

Total RNA Purification Kit - Safety Data Sheet

Total RNA Purification 96-Well Kit - Safety Data Sheet

Poster

RNA Quality Assessment and The Effect On Gene Expression Studies - ASCB 2008

Substrate Specificity of Taq Polymerase - ASCB 2007

Genomic Profiling of Hepatitis B Virus and Hepatitis C Virus From 1 mL of Urine - ASM 2007

Silicon Carbide As A Novel RNA Affinity Medium With Improved Sensitivity and Size Diversity - microRNA 2008

Effect of RNA Isolation Methods On microRNA Quantity and Quality In Plasma - TriCon 2013

Revised Guidelines For RNA Quality Assessment For Diverse Biological Sample Input - ASCB 2008

Variation Between The Purification Efficiency of microRNA With Different Gc Content - TriCon 2013

The Impact of Sample Preparation On Microrna Quality and Diversity For Downstream Applications - microRNA 2009

Rapid and qPCR ready DNA isolation from environmental and human bodily fluid samples using Magnetic bead system

Product Flyers

Total RNA Purification

RNA Clean-Up and Concentration Micro-Elute Kit