Description
Propidium Iodide (PI) is a membrane-impermeant DNA binding dye that cannot penetrate viable cells. PI rapidly enters cells with compromised membranes and intercalates between base pairs allowing exclusion of non-viable cells from analysis of flow cytometry data. PI has a broad emission spectrum from 535-617 nm and can be detected in either FL2 or FL3 detectors. When used with Annexin V FITC, it is recommended to analyze PI in FL2.
Name |
Propidium Iodide |
---|---|
Cat. No. |
13-6990 |
Excitation Laser |
Blue (488 nm), Green (532 nm), Yellow-Green (561 nm) |
Emission (nm) |
535 - 617 |
Formulation |
10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2 |
Application |
Flow Cytometry |
Catalog #: 13-6990-T200 -- 200 tests
Catalog #: 13-6990-T500 -- 500 tests
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