RayBiotech Human/Mouse/Rat Phospho-GSK3b (S9) ELISA
Phosphorylation ELISA. Catalog #: PEL-GSK3b-S9
Human/Mouse/Rat Phospho-GSK3b (S9) ELISA
RayBio® Human Phospho-GSK3b (S9) ELISA Kit. This assay semi-quantitatively measures phosphorylated GSK3b (Ser9) in lysate samples.
Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
---|---|
Species | Human, Mouse, Rat |
Quantitative/Semi-Quantitative | Semi-Quantitative |
Protein Name / Synonyms | Glycogen synthase kinase-3 beta (GSK-3 beta) (EC 2.7.11.26) (Serine/threonine-protein kinase GSK3B) (EC 2.7.11.1) |
Specificity | The antibody pair provided in this kit recognizes human, mouse and rat Phospho-GSK3b (pSer9). |
Accession Number | P49841 |
Gene Symbols | GSK3B |
Gene Id | 2932 |
Compatible Sample Types | Tissue Lysates, Cell Lysates |
Solid Support | 96-well Microplate |
Method Of Detection | Colorimetric |
Design Principle | Sandwich-based |
Research Area | Post-Translational Modifications, Phosphorylation, mTOR Signaling, PI3K-AKT Signaling, Insulin Signaling |
RayBio? Phospho-GSK3beta (S9) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining phosphorylated GSK3beta protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human/mouse/rat phosphoGSK3beta. An anti-pan GSK3beta antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and GSK3beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-GSK3beta (S9) antibody is used to detect phosphorylated GSK3beta. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GSK3beta (S9). The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Kit Components
Other Materials Required
Protocol Outline
Positive Control
NIH 3T3 cells were treated with recombinant human PDGF BB at 37oC for 10 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.
Recombinant Human PDGF BB Stimulation of NIH 3T3 Cell Lines
NIH 3T3 cells were treated or untreated with 50 ng/ml recombinant human PDGF BB for 10 min. Cell lysates were analyzed using this phosphoELISA and Western Blot.
Upon receipt, the kit should be stored at –20°C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4°C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge, and store at –20°C. Item D, store at 2-8°C for up to one month (store at -20°C for up to 6 months, avoid repeated freeze-thaw cycles). Reconstituted Positive Control (Item K) should be stored at -70°C.
Catalog #: PEL-GSK3b-S9-1 -- 1 Plate Kit
Catalog #: PEL-GSK3b-S9-2 -- 2 Plate Kit
Catalog #: PEL-GSK3b-S9-5 -- 5 Plate Kit
Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.