Adding Image Analysis to Gentle Microfluidic Cell Sorting

Spatial Information

Captures images of each cell. In addition to fluorescence intensity, the VERLO provides spatial information such as cell morphology and marker localization

Enhanced Resolution

Imaging of subcellular structures and analysis of spatial variations in brightfield, darkfield, and fluorescent images

Increased Precision Sorting

Following the acquisition and processing of cell images, imaging features are incorporated into the gating strategy to define cell phenotypes

Multivariate Datasets

Combines high-throughput sampling with single-cell image acquisition and measures large number of features from cell images, providing more comprehensive data

Key VERLO™ Applications

VERLO™ Image Sorting Technology 12-Parameter Image-Guided Sorting

With 488 nm and 561 nm lasers to detect up to 9 fluorescent markers and 3 channels of label-free information, the VERLO Image-Guided Cell Sorter can identify key structures, such as golgi and nuclei, internalization, overall morphology, and nuanced texture features that can discriminate live-dead—as well as the physiology of the cells.

Once you have decided on which morphological features of the cells you want to sort, you can gate on image features such as Area vs Max Intensity, just like you would when sorting using FSC vs BSC. The image features are calculated in real-time, enabling you to begin sorting your samples without having to first analyze the whole sample and then run it through the instrument again.

Microfluidics

The VERLO™ Image-Guided Cell Sorter uses patented, microfluidic-based sorting with robust laser-excitation and sensitive PMT detectors to isolate mammalian cells, microbes, plant cells and more. A gentle and precise piezoelectric actuator directs cells into collection channels and allows analysis and sorting in a disposable format. This eliminates sample-to-sample contamination and biohazard exposure or cleanup. This technology allows the VERLO™ to sort up to 200 cells per second with high accuracy and effective recovery. Deposit 1 to 100 cells per well in a 96- or 384 well plate using a single-cell sorting cartridge and integrated Single-Cell Dispenser. CellVantage Software VERLO™ integrated platform uses patented, microfluidic-based sorting with robust laser-excitation and sensitivePMT detectors to isolate mammalian cells, microbes, plant cells and more. Agentle and precise piezoelectric actuator directs cells into collection channels and allows analysis and sorting in a disposable format. This eliminates sample-to-sample contamination and biohazard exposure or cleanup.

- Unique to NanoCellect are our disposable cartridges that allow for bulk sorting or single-cell sorting. 

- The sorting cartridges use a piezoacoustic actuator that gently directs cells into collection channels; an embedded cell sorting verification system gives instant feedback of sorting accuracy. 

- This technology allows the VERLO™ to sort up to 200 cells per second with high accuracy and effective recovery. 

- Sort two selected cell populations with bulk sorting while the remainder of cells collects in a third channel. 

- Deposit 1 to 100 cells per well in a 96- or 384 well plate using a single-cell sorting cartridge and integrated Single-Cell Dispenser.

VERLO™ Specifications

Fluidics

Plate Sorting Specifications

Performance

Optics

Instrument Specifications

VERLO™ Configuration

The VERLO's dual laser configuration allows 9 fluorescent and 3 label-free flow cytometry and imaging channels to be collected.

Lasers and Filters

Optics

Dye Compatability

VERLO™ Integrated Platform

VERLO's integrated system offers seamless compatibility and streamlined workflows, providing researchers with a comprehensive solution for their cell sorting needs.

CellVantage™ Software

The CellVantage software combines the classic flow cytometry features familiar to flow cytometry scientists with an interactive image gallery to visualize all analyzed and sorted cells. The software has an intuitive workflow interface that walks the user through the experimental process and is designed for both novice and expert users.

Single-Cell Dispenser

Designed to sort and dispense into 96- and 384-well plates, the integrated dispenser provides higher rates of singlet detection comparted to cell printers or limiting dilution. Users can preform simple, label-free dispensing or advanced multicolor panel single-cell dispensing.

Plate sorting specifications:

- Time to Plate (96-well): 6 min
- Time to Plate (384-well): 20 min
- Dispensed Volume: 4 µL
- Plating options: 96-or 384-well plates (flat/U/V bottom or PCR)

Sample Cooling and Mixing

The VERLO™ has integrated sample cooling designed to keep your temperature-sensitive samples cooled and agitated to maintain them in uniform suspension while running bulk and single-cell sorting. Both your sample and sheath fluid are kept at the desired temperature. In addition, sorted cells are also kept cooled whether you sort in tube or multi-well plates.

Sample cooling and mixing specifications:

- Chilling Temperature: 4-24°C (39.2-75.2°F) 
- Sample Rotation Speed: 5-100 rpm

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.