Overview

Culture, differentiate, and mature neurons derived from human embryonic stem (ES) or induced pluripotent stem (iPS) cells by using a complete medium optimized to promote, rather than inhibit neuronal activity.

For your convenience, BrainPhys™ hPSC Neuron Kit includes serum-free BrainPhys™ Neuronal Medium (basal medium), supplements, and growth factors to enable you to generate and mature different neuronal subtypes from human ES/iPS cell-derived neural progenitor cells. Based on the formulation by Bardy and Gage (Bardy et al. PNAS, 2015), BrainPhys™ Neuronal Medium mimics the extracellular environment of the central nervous system (CNS) to yield a higher proportion of synaptically active neurons. Brewer’s B27-based (Brewer et al. J Neurosci Res., 1993) NeuroCult™ SM1 Neuronal Supplement ensures cell health and encourages neurite outgrowth and branching in short- and long-term serum-free cultures, and N2 Supplement-A supports the in vitro differentiation of ES/iPS-derived cells to neuronal subtypes. Included BDNF and GDNF growth factors support lineage-specific differentiation.

To avoid shocking your cells with media changes, you can also use BrainPhys™ medium when performing functional assays, such as microelectrode array-based recordings or live-fluorescent imaging.

Subtype
Basal Media, Specialized Media
 

Cell Type
Neural Cells, PSC-Derived, Neurons, Pluripotent Stem Cells
 

Species
Human
 

Application
Cell Culture, Differentiation, Maintenance
 

Brand
BrainPhys
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Stem Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- More representative of the brain’s extracellular environment

- Improved neuronal function and a higher proportion of synaptically active neurons

- Perform functional assays without changing media and shocking cells

- Supports long-term culture of ES/iPS cell- and CNS-derived neurons

- Rigorous raw material screening and quality control ensure minimal lot-to-lot variability

What's Included

- BrainPhys™ Neuronal Medium, 500 mL (Catalog #05790)

- NeuroCult™ SM1 Neuronal Supplement, 10 mL (Catalog #05711)

- N2 Supplement-A, 5 mL (Catalog #07152)

- Human Recombinant BDNF, 10 µg (Catalog #78005)

- Human Recombinant GDNF, 10 µg (Catalog #78058)

Catalog #: 05795

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.