Overview

Culture primary or human pluripotent stem cell (hPSC)-derived neurons long term in a medium optimized to promote, rather than inhibit neuronal activity and maturity.

For your convenience, BrainPhys™ Neuronal Medium and SM1 Kit includes both serum-free BrainPhys™ Neuronal Medium (basal medium) and NeuroCult™ SM1 Neuronal Supplement, a Brewer's B27-based supplement (Brewer et al. J Neurosci Res., 1993) that ensures cell health and encourages neurite outgrowth and branching in short- and long-term serum-free cultures. Based on the formulation by Bardy and Gage (Bardy et al. PNAS, 2015), BrainPhys™ Neuronal Medium mimics the extracellular environment of the central nervous system (CNS) to yield a higher proportion of synaptically active neurons.

Use BrainPhys™ Neuronal Medium and SM1 Kit to culture primary CNS-derived neurons or embryonic stem (ES) and induced pluripotent stem (iPS) cell-derived neurons. To avoid shocking your cells with media changes, you can also use this culture system when performing functional assays, such as microelectrode array-based recordings or live-fluorescent imaging.

Subtype
Basal Media, Specialized Media
 

Cell Type
Neural Cells, PSC-Derived, Neurons, Pluripotent Stem Cells
 

Species
Human, Mouse, Rat
 

Application
Cell Culture, Differentiation, Maintenance
 

Brand
BrainPhys
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Stem Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- More representative of the brain’s extracellular environment

- Improved neuronal function and a higher proportion of synaptically active neurons

- Perform functional assays without changing media and shocking cells

- Supports long-term culture of ES/iPS cell- and CNS-derived neurons

- Rigorous raw material screening and quality control ensure minimal lot-to-lot variability

What's Included

- BrainPhys™ Neuronal Medium, 500 mL (Catalog #05790)

- NeuroCult™ SM1 Neuronal Supplement, 10 mL (Catalog #05711)

Catalog #: 05792

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.