Overview

Promote, rather than inhibit, neuronal activity and maturity in your cultured primary or human pluripotent stem cell (hPSC)-derived neurons in a phenol red-free environment.

Based on the formulation by Bardy and Gage (Bardy C et al. PNAS, 2015), BrainPhys™ serum-free neuronal basal medium is optimized to yield a higher proportion of synaptically active neurons by mimicking the central nervous system extracellular environment. Use BrainPhys™ Without Phenol Red to culture primary neurons, differentiate and mature hPSC-derived neurons, record microelectrode array-based neuronal activity, live-fluorescent image neurons, and transdifferentiate somatic cells to neurons without hormonal signaling.

To ensure cell survival in long-term serum-free culture, BrainPhys™ Without Phenol Red must be combined with an appropriate serum-replacement supplement, such as NeuroCult™ SM1 Neuronal Supplement (Catalog #05711) and/or N2 Supplement-A (Catalog #07152).

Subtype
Basal Media, Specialized Media
 

Cell Type
Neural Cells, PSC-Derived, Neurons, Pluripotent Stem Cells
 

Species
Human, Mouse, Rat
 

Application
Cell Culture, Differentiation, Maintenance
 

Brand
BrainPhys
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Stem Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- More representative of the brain’s extracellular environment

- Improved neuronal function and a higher proportion of synaptically active neurons

- Perform functional assays without changing media and shocking cells

- Supports long-term culture of ES/iPS cell- and CNS-derived neurons

- Rigorous raw material screening and quality control ensure minimal lot-to-lot variability

Catalog #: 05791

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.