Overview

ClonaCell™-CHO ACF is a methylcellulose-based semi-solid medium with supplements for the selection and cloning of suspension-adapted CHO cells in serum-free conditions. This medium is animal component-free and contains only recombinant proteins and synthetic components. Single-cell cloning efficiency of CHO cells is higher in this medium than in protein-free media, especially at low plating densities. It does not contain L-glutamine, selection agents, or phenol red and is compatible with dihydrofolate reductase (DHFR) and glutamine synthase (GS) selection systems.

Benefits of semi-solid cloning:
- Individual cells are suspended in viscous medium and form physically separated, discrete colonies that are easily isolated.
- Monoclonal cell lines are isolated in less time using fewer resources compared with selection and cloning by limiting dilution.
- Diverse clones with a wide range of growth rates and productivities form discrete colonies in the viscous medium. As a result, rare and high-producing clones can be individually isolated more easily using simultaneous selection and cloning in semi-solid medium compared with selection in bulk liquid cultures.

Contains
- Methylcellulose
- Recombinant proteins (animal component-free)
- Other ingredients
 

Subtype
Semi-Solid Media, Specialized Media
 

Cell Type
CHO Cells
 

Species
Other
 

Application
Cell Culture, Semi-Solid Cloning
 

Brand
ClonaCell
 

Area of Interest
Antibody Development, Cell Line Development, Drug Discovery and Toxicity Testing
 

Formulation Category
Animal Component-Free, Serum-Free

Product Advantages

- Animal component-free; Contains only recombinant proteins and synthetic components

- Greater single-cell cloning efficiency of CHO cells compared with chemically defined, protein-free media, particularly at low plating densities

Catalog #: 03816

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.