Overview

ClonaCell™-CHO CD Medium is a methylcellulose-based semi-solid medium recommended for selection and cloning of Chinese hamster ovary (CHO) cells. This medium is chemically defined, protein-free, and animal component-free. It does not contain L-glutamine, selection agents, or phenol red and is compatible with dihydrofolate reductase (DHFR) and glutamine synthetase (GS) selection systems.

Benefits of semi-solid cloning:
- Individual clones and their progeny remain localized together in a semi-solid matrix as they grow to form distinct monoclonal colonies. This prevents the loss of rare, high-producing clones by overgrowth from faster-growing cells, as can occur during selection in a liquid medium, and facilitates the isolation of a diverse set of clones with a wide range of growth rates and productivities to be obtained for downstream screening.
- Colonies obtained from semi-solid medium have a high probability of monoclonality, allowing clonal cell lines to be generated in less time and using fewer resources than with limiting dilution cloning.
- Colonies can be easily picked from the semi-solid medium by manual or robotic methods and dispersed into a liquid growth medium for screening and expansion.

Contains
- Methylcellulose
- Kolliphor® P188
- Other ingredients
 

Subtype
Semi-Solid Media, Specialized Media
 

Cell Type
CHO Cells
 

Species
Other
 

Application
Cell Culture, Semi-Solid Cloning
 

Brand
ClonaCell
 

Area of Interest
Antibody Development, Cell Line Development, Drug Discovery and Toxicity Testing
 

Formulation Category
Animal Component-Free, Chemically Defined, Protein-Free, Serum-Free

Product Advantages

- Enables single-cell cloning and expansion of CHO cells in a chemically defined, protein-free, animal component-free medium

Catalog #: 03815

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.