Overview

ClonaCell™-HY AOF Expansion Medium is an animal origin-free (AOF) and serum-free liquid medium optimized for hybridoma expansion after hypoxanthine, aminopterin, thymidine (HAT) selection. The medium contains hypoxanthine and thymidine (HT) and is used to wean hybridomas off aminopterin used during the selection process. ClonaCell™-HY AOF Expansion Medium promotes robust expansion of hybridomas, adaptation from serum-containing to serum-free media, and stabilizing the viability and antibody productivity of hybridoma cell lines. The majority of hybridomas can be switched directly from serum-containing media to ClonaCell™-HY AOF Expansion Medium without an adaptation step. The absence of serum in this medium facilitates detection and purification of the desired hybridoma-derived antibody without interference from serum-derived immunoglobulins.

This medium has been verified for use with mouse and rat hybridomas and is suitable for expansion of mouse myelomas such as Sp2/0, rat myelomas such as YB2/0, and human myelomas such as U266. In most cases, cryopreserved hybridomas can be thawed directly into ClonaCell™-HY AOF Expansion Medium while maintaining a high level of viability. This medium may be used as a serum-free alternative to ClonaCell™-HY Medium A (Catalog #03801) or ClonaCell™-HY Medium E (Catalog #03805). No materials of animal or human origin are used in the manufacture of this medium or its components, to at least the secondary level of manufacturing.

Contains
- Hypoxanthine and thymidine (HT)
- Gentamicin
- Phenol red
- L-Glutamine and other supplements
- Other ingredients, including recombinant proteins
 

Subtype
Specialized Media
 

Cell Type
Hybridomas
 

Species
Human, Mouse, Rat
 

Application
Cell Culture
 

Brand
ClonaCell
 

Area of Interest
Antibody Development, Cell Line Development, Hybridoma Generation
 

Formulation Category
Serum-Free

Product Advantages

- Animal origin-free

- Absence of serum reduces performance variability in the medium

- Simplifies downstream clone screening and antibody purification (no serum-derived IgG)

Catalog #: 03835

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.