Overview

Perform all the steps in hybridoma development and monoclonal antibody production using the media and reagents included in the ClonaCell™-HY Hybridoma Kit:
- ClonaCell™-HY Medium A for myeloma and hybridoma culture
- ClonaCell™-HY Medium B for hybridoma fusion
- ClonaCell™-HY Medium C for hybridoma fusion recovery
- ClonaCell™-HY Medium D for hybridoma selection and cloning
- ClonaCell™-HY Medium E for hybridoma growth
- ClonaCell™-HY PEG to support hybridoma fusion

The ClonaCell™-HY method uses a methylcellulose-based semi-solid selective medium to combine hybridoma selection and cloning into one step. Individual parental hybridoma clones and their progeny remain localized together in the semi-solid medium as they grow to form distinct colonies. This prevents the loss of rare clones by overgrowth from faster-growing cells, as can occur during selection in a liquid medium. The hybridoma colonies can be easily picked from the semi-solid medium by manual or robotic methods and dispersed into a liquid growth medium for screening and expansion.

This kit has been verified for use in mouse and rat hydridoma development and monoclonal antibody production and reportedly is compatible for production, cloning, and expansion of hybridomas using lymphocytes from a variety of host animals including human, mouse, rat, and hamster. For your convenience, the kit components are also available for purchase individually.

Subtype
Semi-Solid Media, Specialized Media
 

Cell Type
Hybridomas
 

Species
Mouse, Other, Rat
 

Application
Cell Culture, Hybridoma Generation
 

Brand
ClonaCell
 

Area of Interest
Antibody Development, Cell Line Development, Drug Discovery and Toxicity Testing, Hybridoma Generation

Product Advantages

- Time savings: Hybridoma selection and cloning are combined into one step

- Resource savings: Single cell-derived hybridomas form visible discrete colonies in semi-solid medium, and are easy to pick and screen, and transfer to liquid medium for expansion

- Cloning efficiency: Individual cells are suspended and immobilized in semi-solid medium, preventing loss of rare, high-producing clones by overgrowth

What's Included

- ClonaCell™-HY Medium A, 500 mL (Catalog #03801)

- ClonaCell™-HY Medium B, 500 mL (Catalog #03802)

- ClonaCell™-HY Medium C, 100 mL (Catalog #03803)

- ClonaCell™-HY Medium D, 90 mL (Catalog #03804)

- ClonaCell™-HY Medium E, 500 mL (Catalog #03805)

- ClonaCell™-HY PEG, 1.5 mL (Catalog #03806)

Catalog #: 03800

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.