Overview

ClonaCell™‐HY Medium D without HAT is a semi-solid methylcellulose-based medium that does not contain any selection agents such as HAT (hypoxanthine, aminopterin, and thymidine), enabling alternative hybridoma or myeloma selection methods to be used. This product can be used to select and clone myelomas or hybridomas in one step by adding an appropriate selection agent to the medium. Individual parental clones and their progeny remain localized together in the semi-solid matrix as they grow to form distinct colonies. This prevents the loss of rare clones by overgrowth from faster-growing cells, as can occur during selection in a liquid medium, and facilitates the isolation of monoclonal colonies. The colonies can be easily picked from the semi-solid medium by manual or robotic methods and dispersed into a liquid medium for screening and expansion. This serum-containing medium has been verified for use in mouse and rat hybridoma development and reportedly is compatible for production and cloning of myelomas and/or hybridomas using lymphocytes from a variety of host animals including human, mouse, rat, and hamster.

Benefits of semi-solid cloning:
• Individual cells are suspended in viscous medium and form physically separated, discrete colonies that are easily isolated.
• Monoclonal cell lines are isolated in less time using fewer resources compared with selection and cloning by limiting dilution.
• Diverse clones with a wide range of growth rates and productivities form discrete colonies in the viscous medium. As a result, rare and high-producing clones can be individually isolated more easily using simultaneous selection and cloning in semi-solid medium compared with selection in bulk liquid cultures.

Contains
• DMEM
• Methylcellulose
• Serum
• Gentamicin
• 2-Mercaptoethanol
• Phenol red
• L-Glutamine and other supplements
• Other ingredients
 

Subtype
Semi-Solid Media, Specialized Media
 

Cell Type
Hybridomas
 

Species
Human, Mouse, Other, Rat
 

Application
Cell Culture, Hybridoma Generation, Semi-Solid Cloning
 

Brand
ClonaCell
 

Area of Interest
Antibody Development, Cancer, Cell Line Development, Hybridoma Generation
 

Formulation Category
Methylcellulose-Based

Product Advantages

- Time savings: Hybridoma selection and cloning are combined into one step

- Resource savings: Single-cell derived hybridomas form visible discrete colonies in semi-solid medium, and are easy to pick and transfer to liquid medium for screening and expansion

- Cloning efficiency: Individual cells are suspended and immobilized in semi-solid medium, preventing loss of rare, high-producing clones due to overgrowth

- Customizable: Allows user to adapt an existing selection method

Catalog #: 03810

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.