Overview

ClonaCell™-HY Medium E is a serum-containing, nutritionally rich liquid medium optimized for hybridoma expansion after hypoxanthine, aminopterin, thymidine (HAT) selection. The medium contains hypoxanthine and thymidine (HT) and is used to wean hybridomas off aminopterin used during the selection process. This medium has been verified for use in mouse and rat hybridoma development and monoclonal antibody production and reportedly is compatible for production, cloning, and expansion of hybridomas using lymphocytes from a variety of host animals including human, mouse, rat, and hamster. ClonaCell™-HY Medium E has proven effective for subcloning and stabilization of unstable hybridoma cell lines.

Contains
- DMEM
- Serum
- Hypoxanthine and thymidine (HT)
- Gentamicin
- 2-Mercaptoethanol
- Phenol red
- L-Glutamine and other supplements
- Other ingredients
 

Subtype
Specialized Media
 

Cell Type
Hybridomas
 

Species
Mouse
 

Application
Cell Culture, Hybridoma Generation
 

Brand
ClonaCell
 

Area of Interest
Antibody Development, Cell Line Development, Drug Discovery and Toxicity Testing, Hybridoma Generation

Catalog #: 03805

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.