Overview

ClonaCell™-TCS Medium is a serum-containing methylcellulose-based semi-solid medium that can be used for selecting and cloning a variety of suspension-adapted cell lines, including CHO-S and hybridomas. It can also be used for semi-solid cloning of some cell lines that grow adherently in the presence of serum, including CHO-K1, BHK-1, and HEK-293. This medium contains pre-selected fetal bovine serum (FBS) and bovine serum albumin (BSA) and supports robust growth of a wide variety of cell types. ClonaCell™-TCS Medium does not contain selection agents.

Benefits of semi-solid cloning:
- Individual cells are suspended in viscous medium and form physically separated, discrete colonies that are easily isolated.
- Monoclonal cell lines are isolated in less time using fewer resources compared with selection and cloning by limiting dilution.
- Diverse clones with a wide range of growth rates and productivities form discrete colonies in the viscous medium. As a result, rare and high-producing clones can be individually isolated more easily using simultaneous selection and cloning in semi-solid medium compared with selection in bulk liquid cultures.

Contains
- IMDM (Iscove's Modified Dulbecco's Medium)
- Methylcellulose
- Pre-selected serum
- Bovine serum albumin
- 2-Mercaptoethanol
- Phenol red
- L-Glutamine
- Other ingredients
 

Subtype
Semi-Solid Media, Specialized Media
 

Cell Type
CHO Cells, HEK-293, Hybridomas, Other
 

Species
Mouse, Other
 

Application
Cell Culture, Semi-Solid Cloning
 

Brand
ClonaCell
 

Area of Interest
Antibody Development, Cell Line Development, Hybridoma Generation

Product Advantages

- Supports high cloning efficiency and robust colony formation for a variety of cell lines such as: B16F-10, BaF/3, BHK-1, CHO-DG44, CHO-K1, CHO-S, FD-5, HEK-293, Jurkat Daudi, K562, Molt-4, UT-7

Catalog #: 03814

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.