Overview

Generate clonal human pluripotent stem cell (hPSC) lines that maintain their genomic integrity and downstream differentiation potential with this supplement. By using CloneR™2, you can increase the cloning efficiency and survival of human embryonic stem (ES) and induced pluripotent stem (iPS) cells under high-stress conditions, including seeding at low or high densities.

For your gene-editing workflows, add CloneR™2 to improve ES and iPS cell survival following electroporation and during clonal deposition (see data below). It also facilitates the survival and expansion of clonal lines generated by low-density seeding (<25 cells/cm2) or by FACS-sorting cells at 1 cell/well, without requiring single-cell adaptation. You can also use CloneR™2 supplement to increase survival of single cells plated at higher densities, including under stressful conditions such as the post-thaw recovery of ES and iPS cell lines and when creating monolayers ahead of downstream differentiation.

Defined and serum-free, CloneR™2 supplement is compatible for use with TeSR™ ES and iPS cell maintenance media, as well as your choice of cell culture matrix.

Subtype
Supplements
 

Cell Type
Pluripotent Stem Cells
 

Species
Human
 

Application
Cell Culture
 

Brand
CloneR
 

Area of Interest
Cell Line Development, Disease Modeling, Stem Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- More Colonies, Ready Sooner. Improved cloning efficiencies with clones ready for selection days sooner

- Robust and Consistent Cloning. Similar high performance across culture systems and cell lines

- Enhanced Survival. Increased plating efficiency at all densities and after high stress events such as electroporation or thawing

- Straight to Single Cells. No single-cell passage adaptation phase required

Catalog #: 100-0691

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.