Overview

Conditional Reprogramming (CR) Medium is optimized to use with 3T3-J2 Irradiated Feeder Cells (Catalog #100-0353) to expand epithelial cells without genetic modification. Mature epithelial cells have been shown to restore tissue-specific progenitor phenotype that enables proliferation in the presence of CR Medium (Liu X et al. 2012; Liu X et al. 2017). This conditional proliferative capability is deactivated once the medium is removed from the cells (Liu X et al. 2012; Liu X et al. 2017). CR Medium is suitable to propagate primary epithelial cells from healthy or tumor tissues (airway, retina, prostate, breast, intestine, pancreas, liver biliary duct, etc.; Liu X et al. 2012; Liu X et al. 2017; Suprynowicz FA et al.). This product is free of antibiotics and intended for Research Use Only. CR Medium must be supplemented with cholera toxin prior to use. 

Cell Type
Epithelial Cells, Mammary Cells, Prostate Cells
 

Species
Human
 

Application
Cell Culture, Differentiation, Expansion
 

Area of Interest
Epithelial Cell Biology, Stem Cell Biology

Catalog #: 100-0352

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.