Overview

Improve derivation efficiency and expansion rates for long-term culture of proliferative human primary keratinocytes using BPE- and serum-free DermaCult™ Keratinocyte Expansion Medium and a feeder-free protocol. More efficient cell expansion means that you can perform repeats and technical replicates from the same sample and avoid purchasing additional undifferentiated cells or sourcing patient-derived samples.

Unlike other commercial expansion media, DermaCult™ Keratinocyte Expansion Medium enables you to obtain > 50 population doublings of human neonatal keratinocytes and > 35 population doublings of human adult keratinocytes. With the flexibility to change the media every two days and cryopreserve proliferative cells at any passage—while maintaining their differentiation potential—you can carry out your research at your convenience. Cells expanded in this medium exhibit cobblestone morphology and express key basal cell markers (K14, p63, ITAG6, and ITGB1) representative of basal keratinocytes and are suitable for differentiation to air-liquid interface (ALI) cultures. 

Formulated without serum or BPE to maximize experimental reproducibility, you can confidently use keratinocytes cultured in DermaCult™ for your in vitro models to study skin and inflammatory diseases, perform irritation and corrosion assays, or to assess the efficacy of cosmetics or drugs. DermaCult™ Keratinocyte Expansion Medium is compatible for passaging with ACCUTASE™ (Catalog #07920) at 3 - 5 day intervals. It is also suitable for use with collagen (e.g. Catalog #04902), such as to establish keratinocyte culture from skin tissue.

Subtype
Specialized Media, Supplements
 

Cell Type
Dermal Cells, Epithelial Cells, Keratinocytes
 

Species
Human
 

Application
Cell Culture
 

Brand
DermaCult
 

Area of Interest
Cancer, Disease Modeling, Drug Discovery and Toxicity Testing, Transplantation Research
 

Formulation Category
Serum-Free

Product Advantages

- Obtain more population doublings of keratinocytes compared to other commercial media

- Improve derivation efficiency from patient samples

- Flexibility to cryopreserve proliferative cells at any passage

- Keratinocytes maintain their differentiation potential even at late passages

- Maximize experimental reproducibility with a defined, serum- and BPE-free formulation

What's Included

- DermaCult™ Keratinocyte Expansion Basal Medium, 490 mL

- DermaCult™ Keratinocyte Expansion Supplement (50X), 10 mL

Catalog #: 100-0500

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.