EpiCult™-B (mouse) is a serum-free liquid culture medium optimized for the culture of mouse mammary luminal and myoepithelial cells. It is ideal for the culture and evaluation of mouse mammary epithelial progenitors in the mammary colony-forming unit assay when used in conjunction with an irradiated feeder layer such as NIH 3T3 cells. This medium is also used for enzymatic dissociation of mouse mammary tissue when supplemented with collagenase and hyaluronidase. Addition of Human Recombinant EGF (Catalog #78006), Human Recombinant bFGF (Catalog #78003), and Heparin Solution (Catalog #07980) is required for culturing cells.
Subtype
Specialized Media
Cell Type
Mammary Cells
Species
Mouse
Application
Cell Culture, Colony Assay
Brand
EpiCult
Area of Interest
Epithelial Cell Biology
Formulation Category
Serum-Free
- EpiCult™-B Basal Medium (Mouse), 450 mL
- EpiCult™-B Proliferation Supplement (Mouse), 50 mL
Catalog #: 05610
Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.