Overview

Improve the genetic stability of human pluripotent stem cell (hPSC) cultures when performing routine single-cell passaging by using eTeSR™, an enhanced feeder-free culture medium. eTeSR™ builds upon previous formulations of TeSR™ (Ludwig et al. Nat Biotechnol, 2006; Ludwig et al. Nat Methods, 2006), the most widely published feeder-free cell culture media for hPSCs.

Specifically developed for single-cell applications, which typically involve shorter passaging schedules at high density, eTeSR™ can be used for routine maintenance, gene editing, or cloning workflows. Use eTeSR™ to produce high-quality hPSCs with improved genetic stability for these applications; stabilization of the components (e.g. FGF2), optimized metabolites, and improved buffering capacity address the increased metabolic demand and cell stress associated with single-cell passaging. Expect equivalent high performance when using eTeSR™ with either daily or restricted feeding schedules.

eTeSR™ is compatible with a variety of cell culture matrices, including Corning® Matrigel® hESC-Qualified Matrix (Corning Catalog #354277) and CellAdhere™ Laminin-521 (Catalog #200-0117).

Each lot of eTeSR™ 10X Supplement is quality-tested in a culture assay using hPSCs.

Subtype
Specialized Media
 

Cell Type
Pluripotent Stem Cells
 

Application
Cell Culture, Expansion, Maintenance
 

Brand
TeSR
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Stem Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- Increase cell yield while reducing the stress associated with single-cell passaging or high-density cultures

- Support cell quality while allowing for alternative feeding schedules with enhanced buffering, stabilization of key components (including FGF2), and optimized metabolites

- Complete your workflow with compatible gene editing, cloning, differentiation, and cryopreservation protocols

- Eliminate spontaneous differentiation

What's Included

-  eTeSR™ Basal Medium, 450 mL

-  eTeSR™ 10X Supplement, 50 mL

Catalog #: 100-1215

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.