Overview

Screen large numbers of novel drug compounds for their effect on the proliferation and myeloid-specific differentiation of human hematopoietic stem and progenitor cells (HSPCs).

Compared to using the colony-forming unit (CFU) assay, the liquid culture-based HemaTox™ assay is more flexible: you can add test compounds at various points throughout the culture and measure their effects on more primitive or more mature progenitor cells. The HemaTox™ Myeloid Kit promotes the proliferation of CD34+ HSPCs and their differentiation into cells expressing myeloid markers such as CD13, CD14, and CD15.

Users can choose the readout method to quantify the response and estimate the IC₅₀ and IC₉₀ of each compound tested. Test up to 160 conditions—in triplicate—per kit in 5 x 96-well plates. This kit may be used on its own or in combination with HemaTox™ Erythroid Kit (Catalog #09701) or HemaTox™ Megakaryocyte Kit (Catalog #09707) to assess lineage-specific drug toxicity in parallel.

Subtype
Specialized Media
 

Cell Type
Hematopoietic Stem and Progenitor Cells, Myeloid Cells
 

Species
Human
 

Application
Cell Culture, Toxicity Assay
 

Brand
HemaTox
 

Area of Interest
Drug Discovery and Toxicity Testing, Stem Cell Biology
 

Formulation Category
Serum-Free

What's Included

- HemaTox™ Myeloid Medium, 100 mL

- HemaTox™ Myeloid 100X Supplement, 1 mL

Catalog #: 09704

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.