Overview

HepatiCult™ Organoid Growth Medium (Mouse) is a serum-free, defined cell culture medium for establishment and maintenance of mouse hepatic progenitor organoids. These organoids, or "mini-livers", provide an in vitro organotypic culture system for studying hepatic stem and progenitor cells. Organoids grown in HepatiCult™ feature an epithelium expressing genes marking hepatic stem and progenitor cells (PROM1, AXIN2, SOX9 and CD44), ducts (KRT19 and HNF1b) and hepatocytes (HNF4a, AFP). Hepatic organoids can be passaged every 4 - 7 days, can be cryopreserved, and are primed for downstream differentiation.

HepatiCult™ supports mouse hepatic organoid culture either embedded in Corning® Matrigel® domes or in a dilute Matrigel® suspension. Organoid culture enables convenient in vitro characterization of the hepatic epithelium in a physiologically relevant system and reduces the need for animal use.

Subtype
Specialized Media
 

Cell Type
Hepatic Cells
 

Species
Mouse
 

Application
Cell Culture, Expansion, Maintenance, Organoid Culture
 

Brand
HepatiCult
 

Area of Interest
Cancer, Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Stem Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- Convenient in vitro system for generating organoids in 4 - 5 days

- Step-by-step protocol with no injury models, hand-picking of ducts, or cell sorting required

- Simple, two-component format; serum-free and defined medium formulation

- Flexible protocol for generation and maintenance of organoid cultures in matrix domes or suspension culture

What's Included

- HepatiCult™ OGM Mouse Basal Medium, 95 mL

-HepatiCult™ OGM Mouse Supplement, 5 mL

Catalog #: 06030

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.