Overview

IntestiCult™ Organoid Differentiation Medium (Human) is a complete culture medium that supports the further differentiation of intestinal organoids in three dimensions (3D), or in 2D as monolayers or air-liquid interface (ALI) cultures. The starting cultures can be either intestinal organoids derived from human intestinal crypts, or passaged organoids that have been cultured with IntestiCult™ Organoid Growth Medium (Human; Catalog #06010).

Intestinal cultures generated using IntestiCult™ Organoid Differentiation Medium (Human) contain physiologically relevant proportions of differentiated and stem cell populations, recapitulating the diversity of the crypt-villus axis. When compared to conventional cell lines, intestinal monolayers exhibit greater barrier integrity, express higher levels of key differentiation markers, and have a morphology that is more representative of the in vivo intestine.

Applications of intestinal organoid cultures include studying the development and function of the intestinal epithelium, modeling intestinal diseases, compound screening, and regenerative therapy approaches. Intestinal monolayer and ALI cultures are particularly amenable for permeability assays and studies of infectious diseases due to easy access to the apical surface. This kit requires IntestiCult™ Organoid Growth Medium (Human; Catalog #06010) for the initiation and expansion of intestinal organoids prior to differentiation.

Subtype
Specialized Media
 

Cell Type
Intestinal Cells
 

Species
Human
 

Application
Cell Culture, Differentiation, Expansion, Maintenance, Organoid Culture
 

Brand
IntestiCult
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Stem Cell Biology

Product Advantages

- Enables generation of intestinal organoid cultures with physiological proportions of differentiated and stem cell populations

- Allows easy access to the apical surface by transitioning intestinal organoids into monolayer and ALI cultures

- Generates consistent results across passages and replicates

What's Included

- IntestiCult™ ODM Human Basal Medium, 50 mL

- Organoid Supplement, 50 mL

Catalog #: 100-0214

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.