Overview

IntestiCult™ Organoid Growth Medium (Mouse) is a defined, serum-free cell culture medium for efficient establishment and long-term maintenance of mouse intestinal organoids.These organoids, or “mini-guts”, provide a convenient in vitro organotypic culture system for studying both the small and large intestinal epithelium and associated stem cell dynamics. Organoids grown in IntestiCult™ feature a polarized epithelium that contains all of the known cell types of the adult intestinal epithelium. Individual intestinal crypts rapidly form organoids when cultured in IntestiCult™ Organoid Growth Medium (Mouse). Applications of these cultures include studying the development and function of the normal and tumorigenic intestinal epithelium, modeling intestinal disease, and investigating stem cell properties and regenerative therapy approaches. Organoid culture enables convenient in vitro characterization of a system with strong physiological relevance to the adult intestine.

Subtype
Specialized Media
 

Cell Type
Intestinal Cells
 

Species
Mouse
 

Application
Cell Culture, Differentiation, Expansion, Maintenance, Organoid Culture
 

Brand
IntestiCult
 

Area of Interest
Cancer, Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Stem Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- Convenient, in vitro system that recapitulates the identity and organization of the adult intestinal epithelium, including intra- and intercellular signaling, self-propagating stem cell niche and functional transport into and out of the lumen

- Serum-free and defined medium formulation that delivers consistent results

- Enables generation of intestinal organoids in less than one week

- Simple format and easy-to-use protocol

What's Included

- IntestiCult™ OGM Mouse Basal Medium, 90 mL

- IntestiCult™ OGM Mouse Supplement 1, 5 mL

- IntestiCult™ OGM Mouse Supplement 2, 5 mL

Catalog #: 06005

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.