Overview

Limiting Dilution Analysis (LDA) has been applied to the in vitro quantitation of hematopoietic stem cells (HSCs) using assays such as the long term culture-initiating cell (LTC-IC) assay (Conneally et al.) and the cobblestone area forming cell (CAFC) assay (Wang et al.). Limiting dilution protocols have also been adapted to allow the quantitation of human and mouse HSCs with the capacity to produce mature cells of all hematopoietic lineages in vivo, in competitive repopulating unit (CRU) (Ploemacher et al.; Szilvassy et al.) and SCID repopulating cell (SRC) assays (Dick et al.).

L-Calc™ will assist you in analyzing the results of your LDA. For each experiment, values corresponding to dose (i.e. cells per well); total number of positive responses obtained per dose; and total number of replicates tested (i.e. total number of wells used per cell dose) are entered. From these data, the frequency of cells within a given cell population capable of a defined response is determined. L-Calc™ can also be used as a guide to assess differences between paired sets of LDAs. A statistician should be consulted to perform the appropriate comparison.

Free Software Download Available: lcsetup.exe

Suitable for use with Microsoft® Windows.
Please note we no longer provide support or updates for L-Calc software.

Application
Functional Assay
 

Area of Interest
Stem Cell Biology

Catalog #: 28600

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.