Overview

MesenCult™ Expansion Kit (Mouse) is standardized for the culture of mouse mesenchymal stromal cells (MSCs; also known as mesenchymal stem cells) and mouse embryonic fibroblasts (MEFs). The kit includes MesenCult™ Basal Medium (Mouse), MesenCult™ 10X Supplement (Mouse), and MesenPure™. MesenCult™ Expansion Medium has been optimized for the derivation and expansion of mouse MSCs and MEFs in vitro as well as for the detection of colony-forming unit–fibroblasts (CFU­F). This kit was optimized using cells from the mouse strain C57BL/6.
To facilitate the enrichment of MSCs and MEFs during cell culture without serial passaging and frequent medium changes, simply add MesenPure™ to complete MesenCult™ Expansion Medium just prior to use. Although not required, the addition of MesenPure™ is strongly recommended, as the resulting MSC and MEF cultures are more homogeneous and exhibit more robust proliferation, differentiation, and colony formation when compared to complete MesenCult™ Expansion Medium alone.
NOTE: MesenCult™ Expansion Medium must be supplemented with L-Glutamine (Catalog #07100).

Subtype
Specialized Media
 

Cell Type
Mesenchymal Stem and Progenitor Cells, Mouse Embryonic Fibroblasts
 

Species
Mouse
 

Application
Cell Culture, Colony Assay, Expansion
 

Brand
MesenCult
 

Area of Interest
Stem Cell Biology

Product Advantages

- Fast expansion of mouse MSCs with robust enrichment as early as passage 0.

- Optimized for use with mouse bone marrow-, compact bone- and adipose-derived MSCs and MEFs.

- Obtain homogeneous mouse MSC cultures while maintaining tri-lineage differentiation potential.

- Rigorous raw material screening and quality control minimize lot-to-lot variability and increase reproducibility between experiments.

What's Included

- MesenCult™ Basal Medium (Mouse), 450 mL

- MesenCult™ 10X Supplement (Mouse), 50 mL

- MesenPure™, 0.5 mL

Catalog #: 05513

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.