Overview

MesenCult™-ACF Chondrogenic Differentiation Medium is animal component-free (ACF) and specifically formulated for the in vitro differentiation of primary human mesenchymal stromal cells, and hPSC-derived mesenchymal progenitor cells (MSCs; also known as mesencyhymal stem cells) into chondrogenic lineage cells, including chondrocytes. This medium is suitable for the differentiation of human bone marrow (BM)-, adipose tissue (AT)- and synovium (S)-derived MSCs previously culture-expanded in serum-containing medium (e.g. MesenCult™ Proliferation Kit [Catalog #05411]) or animal component-free MesenCult™-ACF Plus Medium [Catalog #05445]). MesenCult™-ACF Chondrogenic Differentiation Medium induces robust chondrogenic differentiation of human MSCs with as few as 3 x 10^5 cells and as early as day 14.

Subtype
Specialized Media
 

Cell Type
Chondrocytes, Mesenchymal Stem and Progenitor Cells
 

Species
Human
 

Application
Cell Culture, Differentiation
 

Brand
MesenCult
 

Area of Interest
Stem Cell Biology
 

Formulation Category
Animal Component-Free, Serum-Free

Product Advantages

- Animal component-free (ACF) formulation

- Robust chondrogenic differentiation with as few as 3 x 10^5 MSCs and as early as day 14

- Strong expression of chondrogenic transcripts - Acan, Col2a, Sox9 and Col10a; low expression of hypertrophic transcript Mmp13

- Completes optimized ACF workflow for MSC isolation, expansion, cryopreservation and chondrogenic differentiation

What's Included

- MesenCult™-ACF Chondrogenic Differentiation Basal Medium, 95 mL

- MesenCult™-ACF 20X Chondrogenic Differentiation Supplement, 5 mL

Catalog #: 05455

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.