Overview

Reduce variability in your human mesenchymal stromal cell (MSC; also known as mesenchymal stem cell) cultures and improve experimental reproducibility by using this animal component-free (ACF) and extracellular vesicle (EV)-free medium. MesenCult™-ACF Plus Culture Kit is optimized so you can derive human MSCs from multiple sources, such as bone marrow or adipose tissue, without serum.

Compared to serum-containing or EV-depleted serum-containing media, MSCs cultured with this kit expand more efficiently (rate and cumulative cell total) and without compromising function. The cultured cells exhibit characteristic MSC surface marker expression and retain robust expansion rate and trilineage differentiation capacities.

This kit is part of a complete ACF workflow—for deriving, expanding, and cryopreserving MSCs, as well as differentiating human pluripotent stem cells into mesenchymal progenitors—that is optimized for efficient and consistent MSC cultures.

For animal component-free and optimized cryopreservation, MesenCult™-ACF Freezing Medium is recommended for human MSCs previously cultured in MesenCult™ media, including MesenCult™-ACF Plus. 

NOTE: Complete MesenCult™-ACF Plus Medium must be supplemented with L-Glutamine. The medium and supplement required for preparation of the complete medium is also available for purchase, without the substrate, as MesenCult™-ACF Plus Medium Kit.

CollPlant is the manufacturer of the rhCollagen component of the cell attachment substrate.

The Product may be used for research purposes only.

Subtype
Specialized Media
 

Cell Type
Mesenchymal Cells, PSC-Derived, Mesenchymal Stem and Progenitor Cells
 

Species
Human
 

Application
Cell Culture, Expansion, Maintenance
 

Brand
MesenCult
 

Area of Interest
Extracellular Vesicle Research, Stem Cell Biology
 

Formulation Category
Animal Component-Free, Serum-Free

Product Advantages

- Animal component-free formulation improves experimental reproducibility.

- Superior cell expansion compared to serum-containing media.

- Cultured MSCs retain robust expansion and tri-lineage differentiation capacities at early and late passages.

- Supports MSC derivation directly from primary human tissue.

What's Included

- MesenCult™-ACF Plus Medium, 500 mL

- MesenCult™-ACF Plus 500X Supplement, 1 mL

- Animal Component-Free Cell Attachment Substrate, 1 mL

Catalog #: 05448

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.