Overview

MethoCult™ Express is intended for use in hematopoietic colony-forming unit (CFU) assays of human cord blood (CB) samples, after a minimum culture period of 7 days. It is recommended for use with red blood cell-depleted CB samples, whole CB samples that have been cryopreserved and thawed, and CB mononuclear cells. MethoCult™ Express is optimized for the growth and enumeration of human hematopoietic progenitor cells after much shorter periods than the 14 -16 days of standard CFU assays. In MethoCult™ Express, colonies containing at least 20 cells can be counted as early as after 7 days of culture. At this time, most colonies are immature and have not yet differentiated into morphologically distinguishable colony types. Therefore the colonies counted after 7 days of culture give information about the total frequency of hematopoietic progenitor cells present in the sample without distinction between different progenitor cell types. If MethoCult™ Express cultures are maintained for 14 - 16 days, colonies derived from erythroid progenitor cells (BFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G, and CFU-M), and multi-potential granulocyte, erythroid, macrophage, and megakaryocyte progenitor cells (CFU-GEMM) can be counted.

Contains
- Methylcellulose in Iscove's MDM
- Fetal bovine serum
- Bovine serum albumin
- Cytokines, including erythropoietin (EPO)
- Supplements
 

Subtype
Semi-Solid Media, Specialized Media
 

Cell Type
Hematopoietic Stem and Progenitor Cells
 

Species
Human
 

Application
Cell Culture, Colony Assay, Functional Assay
 

Brand
MethoCult
 

Area of Interest
Cord Blood Banking, Stem Cell Biology, Transplantation Research

Catalog #: 04437 (1 x 100 mL bottle), 04447 (24 x 3 mL)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.