Overview

MethoCult™ GF M3534 is optimized for the growth and enumeration of granulocyte-macrophage progenitor cells (CFU-GM, CFU-G, CFU-M) in colony-forming unit (CFU) assays of mouse bone marrow, spleen, peripheral blood, and fetal liver cells. MethoCult™ M3534 does not support the growth of erythroid progenitor cells (BFU-E and CFU-E) as it does not contain erythropoietin (EPO). This formulation is compatible with STEMvision™ software for automated colony counting of mouse bone marrow CFU assays.

Contains
- Methylcellulose in Iscove's MDM
- Fetal bovine serum
- Bovine serum albumin
- Recombinant human insulin
- Human transferrin (iron-saturated)
- 2-Mercaptoethanol
- Recombinant mouse stem cell factor (SCF)
- Recombinant mouse interleukin 3 (IL-3)
- Recombinant human interleukin 6 (IL-6)
- Supplements
 

Subtype
Semi-Solid Media, Specialized Media
 

Cell Type
Hematopoietic Stem and Progenitor Cells
 

Species
Mouse
 

Application
Cell Culture, Colony Assay, Functional Assay
 

Brand
MethoCult
 

Area of Interest
Drug Discovery and Toxicity Testing, Stem Cell Biology
 

Formulation Category
Methylcellulose-Based

Catalog #: 03534

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.