Overview

MethoCult™ H4034 Optimum (MethoCult™ GF H4034) is a methylcellulose-based medium for the growth and enumeration of hematopoietic progenitor cells in colony-forming unit (CFU) assays of human bone marrow, mobilized peripheral blood, peripheral blood, and cord blood samples. MethoCult™ H4034 Optimum has been formulated to support optimal growth of erythroid progenitor cells (BFU-E and CFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M), and multipotential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells (CFU-GEMM). The inclusion of G-CSF in Optimum formulations improves discrimination of myeloid colony subtypes. This formulation is compatible with STEMvision™ software for automated colony counting.

Contains
- Methylcellulose in Iscove's MDM
- Fetal bovine serum
- Bovine serum albumin
- 2-Mercaptoethanol
- Recombinant human stem cell factor (SCF)
- Recombinant human interleukin 3 (IL-3)
- Recombinant human erythropoietin (EPO)
- Recombinant human granulocyte colony-stimulating factor (G-CSF)
- Recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF)
- Supplements
 

Subtype
Semi-Solid Media, Specialized Media
 

Cell Type
Hematopoietic Stem and Progenitor Cells
 

Species
Human, Non-Human Primate
 

Application
Cell Culture, Colony Assay, Functional Assay
 

Brand
MethoCult
 

Area of Interest
Drug Discovery and Toxicity Testing, Stem Cell Biology

Catalog #: 04034 (1 x 100 mL bottle), 04044 (24 x 3 mL)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.