Overview

MethoCult™ H4330 is a methylcellulose-based medium for the growth and enumeration of hematopoietic progenitor cells in colony-forming unit (CFU) assays of human bone marrow, mobilized peripheral blood, peripheral blood, and cord blood samples. This formulation contains erythropoietin (EPO), but does not contain other cytokines. It is recommended for assays of late erythroid progenitors, specifically colony-forming cell - erythroid (CFU-E), in the presence of EPO only. It is also recommended for assays of early erythroid progenitors, specifically burst-forming unit - erythroid (BFU-E), and other colony-forming cells when appropriate cytokines are added. It can also be used to assess colony-stimulating factor activity or burst-promoting activity in unknown samples.

Contains
- Methylcellulose in Iscove's MDM
- Fetal bovine serum
- Bovine serum albumin
- 2-Mercaptoethanol
- Recombinant human erythropoietin (EPO)
- Supplements
 

Subtype
Semi-Solid Media, Specialized Media
 

Cell Type
Hematopoietic Stem and Progenitor Cells
 

Species
Human, Non-Human Primate
 

Application
Cell Culture, Colony Assay, Functional Assay
 

Brand
MethoCult
 

Area of Interest
Stem Cell Biology

Catalog #: 04330

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.