Overview

MethoCult™ H4431 is a methylcelluose-based medium for the growth and enumeration of hematopoietic progenitor cells in colony-forming unit (CFU) assays of bone marrow, mobilized peripheral blood, peripheral blood, and cord blood samples. MethoCult™ H4431 supports the growth of erythroid progenitor cells (BFU-E and CFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M), and multipotential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells (CFU-GEMM). This formulation contains agar leukocyte conditioned medium (agar-LCM) as a source of colony-stimulating factors, plus recombinant human erythropoietin (EPO). It is suitable for use as a control medium for detection of “EPO-independent” erythroid progenitor cells using MethoCult™ H4531 (Catalog #04531).

Contains
- Methylcellulose in Iscove's MDM
- Fetal bovine serum
- Bovine serum albumin
- 2-Mercaptoethanol
- Agar-LCM
- Recombinant human erythropoietin (EPO)
- Supplements
 

Subtype
Semi-Solid Media, Specialized Media
 

Cell Type
Hematopoietic Stem and Progenitor Cells
 

Species
Human
 

Application
Cell Culture, Colony Assay, Functional Assay
 

Brand
MethoCult
 

Area of Interest
Stem Cell Biology

Catalog #: 04431

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.