Overview

MethoCult™ H4435 Enriched (MethoCult™ GF+ H4435) is a complete methylcellulose-based medium for the growth and enumeration of hematopoietic progenitor cells in colony-forming unit (CFU) assays of human bone marrow, mobilized peripheral blood, peripheral blood, and cord blood samples. This medium supports optimal growth of erythroid progenitor cells (BFU-E and CFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M), and multipotential granulocyte, erythroid, macrophage and megakaryocyte progenitor cells (CFU-GEMM). MethoCult™ H4435 Enriched is recommended for use with CD34+ cells and other purified cell populations, and for CFU assays performed as part of LTC-IC assays.

Contains
- Methylcellulose in Iscove's MDM
- Fetal bovine serum
- Bovine serum albumin
- 2-Mercaptoethanol
- Recombinant human stem cell factor (SCF)
- Recombinant human interleukin 3 (IL-3)
- Recombinant human interleukin 6 (IL-6)
- Recombinant human erythropoietin (EPO)
- Recombinant human granulocyte colony-stimulating factor (G-CSF)
- Recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF)
- Supplements
 

Subtype
Semi-Solid Media, Specialized Media
 

Cell Type
Hematopoietic Stem and Progenitor Cells
 

Species
Human, Non-Human Primate
 

Application
Cell Culture, Colony Assay, Functional Assay
 

Brand
MethoCult
 

Area of Interest
Stem Cell Biology

Catalog #: 04435 (1 x 100 mL bottle), 04445 (24 x 3 mL)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.