Overview

MethoCult™ SF H4636 is recommended for the culture of human embryonic stem (ES) cell-derived and induced pluripotent stem (iPS) cell-derived hematopoietic progenitor cells in defined serum-free conditions. MethoCult™ SF H4636 is formulated to support optimal growth of erythroid progenitor cells (CFU-E and BFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G, CFU-M), and multipotential progenitor cells (CFU-GEMM; granulocyte, erythrocyte, macrophage, megakaryocyte).

MethoCult™ SF H4636 is also recommended for CFU assays with mononuclear cells, CD34+ enriched cells, and cells isolated by other purification methods from human bone marrow (BM), mobilized peripheral blood (MPB), peripheral blood (PB), and cord blood (CB) samples.

Contains
- Methylcellulose in Iscove's IMDM
- Bovine serum albumin
- 2-Mercaptoethanol
- Recombinant human (rh) insulin
- Human transferrin (iron-saturated)
- Supplements
- rh stem cell factor (SCF)
- rh interleukin 3 (IL-3)
- rh granulocyte colony-stimulating factor (G-CSF)
- rh granulocyte-macrophage colony-stimulating factor (GM-CSF)
 

Subtype
Semi-Solid Media, Specialized Media
 

Cell Type
Hematopoietic Stem and Progenitor Cells
 

Species
Human
 

Application
Cell Culture, Colony Assay, Functional Assay
 

Brand
MethoCult
 

Area of Interest
Stem Cell Biology
 

Formulation Category
Serum-Free

Catalog #: 04636

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.