Overview

MethoCult™ SF M3436 is optimized for the growth and enumeration of primitive erythroid progenitor cells (BFU-E) in colony-forming unit (CFU) assays of mouse bone marrow, fetal liver, spleen, peripheral blood, and purified cells. MethoCult™ SF M3436 does not support the growth of granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M) or multipotential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells (CFU-GEMM). This formulation is serum-free and compatible with STEMvision™ software for automated colony counting of mouse bone marrow CFU assays. MethoCult™ SF M3436 can also be used for growth and enumeration of BFU-E in CFU assays of rat bone marrow cells.

Contains
- Methylcellulose in Iscove's MDM
- Bovine serum albumin
- Recombinant human insulin
- Human transferrin (iron-saturated)
- 2-Mercaptoethanol
- Cytokines (including recombinant human erythropoietin [EPO])
- Supplements
 

Subtype
Semi-Solid Media, Specialized Media
 

Cell Type
Hematopoietic Stem and Progenitor Cells
 

Species
Mouse, Rat
 

Application
Cell Culture, Colony Assay, Functional Assay
 

Brand
MethoCult
 

Area of Interest
Drug Discovery and Toxicity Testing, Stem Cell Biology
 

Formulation Category
Serum-Free

Catalog #: 03436

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.