Overview

MyoCult™ Expansion 10X Supplement (Mouse), when combined with DMEM/F-12 to prepare MyoCult™ Expansion Medium, is for the culture of mouse skeletal muscle progenitor cells (satellite cells). MyoCult™ Expansion Medium has been optimized for the expansion of mouse skeletal muscle satellite cells isolated by fluorescence-activated cell sorting (FACS), as well as for culturing single isolated myofibers grown in suspension. Satellite cells cultured using MyoCult™ Expansion Medium can be differentiated into multinucleated myotubes.

For preparation of MyoCult™ Expansion Medium, DMEM/F-12 with 15 mM HEPES (Catalog #36254) is required in addition to MyoCult™ Expansion 10X Supplement. For expansion of satellite cells, cultureware must be coated with a matrix such as Corning Matrigel® Basement Membrane Matrix (Corning Catalog #356234).

Subtype
Specialized Media
 

Cell Type
Myogenic Stem and Progenitor Cells
 

Species
Mouse
 

Application
Cell Culture, Expansion
 

Brand
MyoCult
 

Area of Interest
Stem Cell Biology

Product Advantages

- Supports robust expansion of mouse skeletal muscle progenitor cells.

- Culture-expanded skeletal muscle progenitor cells maintain differentiation potential.

- Rigorous raw material screening and quality control minimize lot-to-lot variability.

Catalog #: 05985

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.