Overview

Culture primary human skeletal muscle progenitor cells and human pluripotent stem cell (hPSC)-derived myoblasts under serum-free conditions.

The MyoCult™-SF Expansion Supplement Kit (Human) includes components optimized for the derivation and expansion of myoblasts in vitro: MyoCult™-SF Expansion 10X Supplement (Human) and MyoCult™-SF Attachment Substrate. The supplement must be combined with a basal medium (DMEM with 1000 mg/L D-Glucose (Catalog #36253); sold separately) to prepare MyoCult™-SF Expansion Medium. Use of the MyoCult™-SF Attachment Substrate is optional depending on the cell source used. For your convenience, the kit components may also be purchased individually.

If you are using primary myoblasts, we recommend coating cultureware with MyoCult™-SF Attachment Substrate. For hPSC-derived myoblasts, you can use Corning® Matrigel® hESC-Qualified Matrix. Myoblasts cultured using MyoCult™-SF Expansion Medium are compatible with MyoCult™ Differentiation Kit (Human) and can be used for further differentiation into multinucleated myotubes.

Subtype
Specialized Media
 

Cell Type
Myogenic Stem and Progenitor Cells
 

Species
Human
 

Application
Cell Culture, Expansion
 

Brand
MyoCult
 

Area of Interest
Stem Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- Supports derivation and long-term expansion of human skeletal muscle progenitor cells under serum-free conditions.

- Culture-expanded skeletal muscle progenitor cells maintain differentiation potential.

- Rigorous raw material screening and quality control minimize lot-to-lot variability.

- Culture-expanded myogenic progenitor cells are compatible with MyoCult™ Differentiation Kit.

What's Included

- MyoCult™-SF Expansion 10X Supplement (Human), 2 x 10 mL

- MyoCult™-SF Attachment Substrate, 100 µg

Catalog #: 05980

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.