Overview

Robustly expand reset naïve human embryonic stem (ES) and induced pluripotent stem (iPS) cells using a defined cell culture medium.

Reset naïve human pluripotent stem cells (hPSCs) cultured in this medium demonstrate uniformly domed, phase-bright colonies with refractive edges and maintain gene expression profiles associated with reset naïve hPSCs (Guo et al. Stem Cell Reports, 2016; Guo et al. Development, 2017; Takashima et al. Cell, 2014). Reset naïve hPSCs cultured in this medium are capable of tri-lineage differentiation into all three somatic lineages following re-priming.

Based on the t2iL + Gö formulation by Austin Smith and developed under license from the Cambridge Stem Cell Institute at the University of Cambridge, UK, this medium is compatible with reset naïve human ES and iPS cells derived using NaïveCult™ Induction Kit (Catalog #05580), transgene-induced reset human pluripotent stem cells (hPSCs) (Takashima et al. 2014), or embryo-derived reset hPSCs (Guo et al. 2016).

Subtype
Specialized Media
 

Cell Type
Pluripotent Stem Cells
 

Species
Human
 

Application
Cell Culture, Expansion, Maintenance
 

Brand
NaïveCult
 

Area of Interest
Stem Cell Biology

Product Advantages

-  Supports robust growth of reset naïve human ES and iPS cells

- Maintains hPSCs with high expression of naïve-associated genes

- Compatible with transgene-independent, transgene-induced, and embryo-derived reset naïve human ES and iPS cells

- Defined formulation containing high-quality, pre-screened components

What's Included

- NaïveCult™ Expansion Basal Medium, 400 mL

- NaïveCult™ 5X Expansion Supplement, 100 mL

- NaïveCult™ 1000X Expansion Supplement, 500 µL

Catalog #: 05590

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.