Overview

Perform transgene-free chemical induction of reset human naïve embryonic stem (ES) and induced pluripotent stem (iPS) cells from the primed state using a defined cell culture medium.

Reset naïve human pluripotent stem cells (hPSCs) generated using this kit demonstrate uniformly domed, phase-bright colonies with refractive edges and maintain gene expression profiles associated with reset naïve hPSCs (Guo et al. Stem Cell Reports, 2016; Guo et al. Development, 2017; Takashima et al. Cell, 2014).

Based on the formulation by Guo et al. (2017) and developed under license from the Cambridge Stem Cell Institute at the University of Cambridge, UK, this kit works robustly across multiple primed human ES and iPS cells maintained in mTeSR™1 (Catalog #85850) or TeSR™-E8™ (Catalog #05990). Note that NaïveCult™ Induction Kit requires a histone deacetylase inhibitor (HDACi; either Sodium Butyrate (Catalog #72242) or Valproic Acid (Catalog #72292)) that is sold separately.

Subtype
Specialized Media
 

Cell Type
Pluripotent Stem Cells
 

Species
Human
 

Application
Cell Culture, Expansion, Maintenance
 

Brand
NaïveCult
 

Area of Interest
Stem Cell Biology

Product Advantages

- Transgene-free chemical induction of reset human naïve ES and iPS cells

- Robust induction across multiple human ES and iPS cell lines

- Generates hPSCs with high expression of naïve-associated genes

- Defined formulation containing high-quality, pre-screened components

What's Included

- NaïveCult™ Induction Basal Medium, 76 mL

- NaïveCult™ 20X Induction Supplement A, 4 mL

- NaïveCult™ 1000X Induction Supplement B, 80 µL

- NaïveCult™ Expansion Basal Medium, 2 x 400 mL

- NaïveCult™ 5X Induction Supplement C, 200 mL

- NaïveCult™ 1000X Induction Supplement D, 500 µL

- NaïveCult™ 1000X Expansion Supplement, 500 µL

Catalog #: 05580

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.