Overview

PancreaCult™ Organoid Growth Medium (Mouse) is a serum-free, defined cell culture medium for establishment and maintenance of mouse pancreatic exocrine organoids. These organoids, or "mini-pancreata", provide an in vitro organotypic culture system for studying pancreatic cell biology, disease, and cancer. Organoids grown in PancreaCult™ feature an epithelium expressing genes marking pancreatic stem cells (LGR5), progenitor cells (PDX1, SOX9), and ductal cells (CAR2, MUC1, KRT19). Pancreatic organoids can be passaged every 3 - 6 days for long-term maintenance and can also be cryopreserved.

PancreaCult™ supports mouse pancreatic organoid culture either embedded in Corning® Matrigel® domes or in a dilute Matrigel® suspension. Organoid culture enables convenient in vitro characterization of the pancreatic epithelium in a physiologically relevant system and reduces the need for animal use.

Subtype
Specialized Media
 

Cell Type
Pancreatic Cells
 

Application
Cell Culture, Expansion, Maintenance, Organoid Culture
 

Brand
PancreaCult
 

Area of Interest
Cancer, Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Organoids, Stem Cell Biology, Transplantation Research
 

Formulation Category
Serum-Free

Product Advantages

- Convenient in vitro system for generating organoids within one week

- Detailed, step-by-step protocol with no injury models, hand-picking of ducts, or cell sorting required

- Simple, two-component format; serum-free and defined medium formulation

- Flexible protocol for generation and maintenance of organoid cultures in matrix domes or suspension culture

What's Included

- PancreaCult™ OGM Mouse Basal Medium, 95 mL

- PancreaCult™ OGM Mouse Supplement, 5 mL

Catalog #: 06040

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.