Overview

Reliably culture pancreatic duct organoids to study the function of the ductal epithelium, model pancreatic diseases such as cancer or cystic fibrosis, and perform targeted drug screens or toxicity assays. With the PancreaCult™ (Human) culture medium system, you have a complete and robust workflow to establish, expand, and maintain pancreatic duct organoids.

Pancreatic organoids can be established from fresh or frozen dissociated tissue in PancreaCult™ Organoid Initiation Medium (OIM), optimized to provide higher rates of organoid formation from tissue. Organoids can then be cultured in PancreaCult™ Organoid Growth Medium (OGM) for long-term maintenance, passaging, serum-free drug screening, or cryopreservation for future experiments. The optional removal of EGF from the medium supports the depletion of normal cells in KRAS-activated cancer organoid cultures.

Organoids grown using the PancreaCult™ (Human) medium system can be adapted to various culture protocols, including two-dimensional Transwell® monolayer cultures, dilute Corning® Matrigel® suspension cultures, and high-throughput assay-compatible culture formats. 

Subtype
Specialized Media
 

Cell Type
Pancreatic Cells
 

Species
Human
 

Application
Cell Culture, Expansion, Maintenance, Organoid Culture
 

Brand
PancreaCult
 

Area of Interest
Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Organoids, Stem Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- Initiate and expand normal and tumor-derived organoids across different donor tissues

- Deplete normal cells from KRAS-activated cancer cells by removing EGF

- Reduce interference and variability in drug screens with a serum-free maintenance medium

- Perform high-throughput drug screening with flexible culture formats

What's Included

- PancreaCult™ Organoid Initiation Medium (Human) (Catalog #100-0820)

    - PancreaCult™ Organoid Basal Medium (Human) , 95 mL

    - PancreaCult™ Organoid Growth Supplement (Human), 5 mL

    - Organoid Supplement, 50 mL

Catalog #: 100-0820

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.