Overview

PneumaCult™ Airway Organoid Kit is a serum- and BPE-free culture medium system for efficient establishment and differentiation of airway organoids derived from human bronchial epithelial cells (HBECs).
After 2D expansion in PneumaCult™-Ex Plus Medium (#05040), PneumaCult™ Airway Organoid Seeding Medium allows for initiation of 3D organoid culture, then PneumaCult™ Airway Organoid Differentiation Medium is used to obtain morphologically relevant airway organoids. Airway organoids provide a functional in vitro organotypic culture system for studying the airway epithelium. When fully differentiated, the organoids exhibit a centralized lumen surrounded by a polarized airway epithelial cell layer, comprised of differentiated cell types, such as ciliated cells and goblet cells. Applications of airway organoid cultures include modeling lung diseases, drug screening for efficacy and toxicity, and studying the functions of the airway epithelium.

Subtype
Specialized Media
 

Cell Type
Airway Cells
 

Species
Human
 

Application
Cell Culture, Differentiation, Expansion, Organoid Culture
 

Brand
PneumaCult
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Organoids, Respiratory Research
 

Formulation Category
Serum-Free

Product Advantages

- Three-dimensional in vitro system that recapitulates key characteristics of the in vivo human airway

- Complete media formulation for expansion and differentiation of human airway epithelial cells, compatible with PneumaCult™-Ex Plus

- Convenient format and easy-to-use protocol that delivers consistent results

What's Included

- PneumaCult™ Airway Organoid Basal Medium, 450 mL (Catalog #05061)

- PneumaCult™ Airway Organoid Seeding Supplement, 10 mL (Catalog #05062)

- PneumaCult™ Airway Organoid Differentiation Supplement, 40 mL (Catalog #05063)

Catalog #: 05060

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.