Overview

Generate apical-out airway organoids in 15 days from human bronchial epithelial cells (HBECs) or human airway epithelial cells (HAECs) with a simple and scalable, Matrigel®-free protocol. These organoids provide access to the apical side of the airway epithelium and can be used to perform your infectious disease modeling or high-throughput drug screening in an in vitro, three-dimensional (3D) model system.

With PneumaCult™ Apical-Out Airway Organoid Medium, you can grow polarized airway organoids with outward-facing, differentiated ciliated cells in AggreWell™400 24-well plates (Catalog #34415)—without the use of cell culture inserts. To maximize experimental reproducibility, this medium is formulated without serum or BPE. For a complete apical-out airway organoid culture workflow, expand your HBECs or HAECs in PneumaCult™-Ex Plus Medium (Catalog #05040) prior to initiating organoid culture with PneumaCult™ Apical-Out Airway Organoid Medium.

Subtype
Specialized Media
 

Cell Type
Airway Cells, Epithelial Cells
 

Species
Human
 

Application
Cell Culture, Differentiation, Organoid Culture
 

Brand
PneumaCult
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Infectious Diseases, Organoids, Respiratory Research, Stem Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- Convenient, in vitro system that provides easy access to the apical side of the airway epithelium

- Easy downstream processing with a Matrigel®-free protocol

- Generates homogeneous organoids across donors

- Suitable for high-throughput drug screening applications

What's Included

- PneumaCult™ Apical-Out Airway Organoid Medium, Catalog #100-0620

    - PneumaCult™ Apical-Out Airway Organoid Basal Medium, 450 mL

    - PneumaCult™ Apical-Out Airway Organoid 10X Supplement, 50 mL

    - PneumaCult™ Apical-Out Airway Organoid 1000X Supplement, 500 µL

Catalog #: 100-0620

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.