Overview

PneumaCult™-ALI Medium (Catalog #05001) is a serum- and BPE-free medium for the culture of human airway epithelial cells at the air-liquid interface (ALI). Airway epithelial cells cultured in PneumaCult™-ALI Medium undergo extensive mucociliary differentiation to form a pseudostratified epithelium that exhibits morphological and functional characteristics similar to those of the human airway in vivo. PneumaCult™-ALI Medium is also available in a kit that includes 12 mm Transwell® inserts (Catalog #05021) or 6.5 mm Transwell® inserts (Catalog #05022).

Together, PneumaCult™-ALI Medium and PneumaCult™-Ex Plus Medium (Catalog #05040) constitute a fully integrated BPE-free culture system for in vitro human airway modeling that is also compatible with primary human nasal epithelial cells. This robust and defined system is a valuable tool for basic respiratory research, toxicity studies, and drug development.

Subtype
Specialized Media
 

Cell Type
Airway Cells
 

Species
Human
 

Application
Cell Culture, Differentiation, Maintenance, Organoid Culture
 

Brand
PneumaCult
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- HBECs cultured with PneumaCult™-ALI undergo extensive mucociliary differentiation to form a pseudostratified epithelium that closely resembles the human airway

- PneumaCult™-ALI is serum-free and BPE-free to minimize variability

What's Included

- PneumaCult™-ALI Basal Medium, 450 mL

- PneumaCult™-ALI 10X Supplement, 50 mL

- PneumaCult™-ALI Maintenance Supplement (100X), 5 x 1 mL

Catalog #: 05001 (Complete Kit), 05022 (Complete Kit with 6.5 mm Inserts), 05021 (Complete Kit with 12 mm Inserts)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.