Overview

ReproRNA™-OKSGM is a single-stranded RNA replicon vector that contains five reprogramming factors: OCT4, KLF-4, SOX2, GLIS1, and c-MYC, as well as a puromycin-resistance gene (Figure 1). This RNA vector reprograms somatic cells, such as fibroblasts, into induced pluripotent stem (iPS) cells with high efficiency and only requires a single transfection step (Figure 2). When used together with ReproTeSR™ reprogramming medium, the generation of iPS cell colonies can be achieved under feeder-free conditions with superior colony morphology and similar reprogramming efficiency to feeder-based systems (Figures 3 - 4). ReproRNA™-derived iPS cell colonies also express markers of undifferentiated cells and retain a normal karyotype (Figures 5-6). Subsequently, iPS cells generated with ReproRNA™-OKSGM can be maintained in TeSR™ maintenance media (mTeSR™1, TeSR™2, or TeSR™-E8™) and further differentiated into cells of all three germ layers (Figure 7).

Subtype
Supplements
 

Cell Type
Pluripotent Stem Cells
 

Species
Human
 

Application
Cell Culture, Reprogramming
 

Brand
TeSR
 

Area of Interest
Stem Cell Biology

Product Advantages

- Non-viral, non-integrating vector system

- Self-replicating vector only requires a single transfection

- Vector contains all reprogramming factors

- Comparable fibroblast reprogramming efficiency to Sendai virus

What's Included

- ReproRNA™-OKSGM Kit (Catalog #05930)

    - ReproRNA™-OKSGM vector (Catalog #05931)

    - ReproRNA™ Transfection Reagent

    - ReproRNA™ Transfection Supplement

    - Recombinant B18R Protein (Catalog #78075)

Catalog #: 05930 (Kit), 05931 (Vector)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.