Overview

Revert primed human embryonic (ES) and induced pluripotent stem (iPS) cells to a naïve-like state and maintain cells in a naïve-like state under hypoxic conditions without bFGF or feeder cells.

Developed under license from the Weizmann Institute of Science, this serum-free cell culture medium produces robust cultures with features of a naïve-like state, such as tightly packed, domed colonies with refractive edges. Human ES/iPS cells cultured in RSeT™ Feeder-Free Medium show increased expression of key transcripts associated with the naïve-like state, such as KLF17, KLF2, KLF4, and TFCP2L1, and can either be differentiated or converted back to a primed state by culture in mTeSR™1 and then differentiated.

Compatible products that can be used for differentiation include STEMdiff™ Definitive Endoderm Kit (Catalog #05110), STEMdiff™ SMADi Neural Induction Kit (Catalog #08581), and STEMdiff™ Mesoderm Induction Medium (Catalog #05220).

Subtype
Specialized Media
 

Cell Type
Pluripotent Stem Cells
 

Species
Human
 

Application
Cell Culture, Expansion, Maintenance
 

Brand
RSeT, TeSR
 

Area of Interest
Stem Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- Feeder-independent culture system reduces inherent variability, cost, and burden of feeder preparation

- Serum-free formulation contains pre-screened quality components for reproducible results

- Facilitates highly efficient reversion to naïve-like state with stable domed morphology, naïve gene expression profiles, and low levels of spontaneous differentiation without the need for exogenous genes

- Maintains naïve-like pluripotency without inclusion of bFGF

What's Included

- RSeT™ Feeder-Free Basal Medium, 450 mL

- RSeT™ Feeder-Free 10X Supplement, 50 mL

Catalog #: 05975

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.