Overview

RSeT™ Medium is a defined cell culture medium used for the reversion of primed human pluripotent stem cells (hPSCs) to a naïve-like state and maintenance of naïve-like hPSCs under feeder-dependent and hypoxic conditions. RSeT™ Medium contains pre-screened quality components and does not contain bFGF or TGFβ. It is compatible with human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells.
hPSCs cultured in RSeT™ Medium exhibit features of a naïve-like state such as tightly packed, domed colonies with refractive edges. Key transcripts associated with naïve-like hPSCs such as KLF2, KLF4, and TFCP2L1 show increased expression in hPSCs cultured in RSeT™ Medium. RSeT™ hPSCs can be converted back to a primed state by culture in mTeSR™1 and can then be differentiated with STEMdiff™ products such as STEMdiff™ Definitive Endoderm Kit (Catalog #05110), STEMdiff™ SMADi Neural Induction Kit (Catalog #08581), and STEMdiff™ Mesoderm Induction Medium (Catalog #05220).

Product Advantages

- Easily passage as single cells while maintaining normal karyotype

- Maintains naïve-like pluripotency without bFGF or TGFβ

- Defined medium contains pre-screened quality components for reproducible results

- No exogenous genes required for reversion to naïve-like state

What's Included

- RSeT™ Basal Medium, 400 mL

- RSeT™ 5X Supplement, 100 mL

Catalog #: 05978

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.