Overview

STEMdiff™ Atrial Cardiomyocyte Differentiation Kit is optimized for the differentiation and maintenance of human embryonic stem and induced pluripotent stem cells into atrial cardiomyocytes (cardiac troponin T-positive; cTnT+). This serum-free kit can be used to generate atrial cardiomyocytes derived from a clump culture of human pluripotent stem cells (hPSCs) maintained in mTeSR™1 (Catalog #85850), mTeSR™ Plus (Catalog #100-0276), or TeSR™-E8™ (Catalog #05990). With greater than 80% yield purity, an average of 1 x 10^6 cTnT+ cells can be harvested from a single well of a 12-well plate.
STEMdiff™ Cardiomyocyte Maintenance Kit (Catalog #05020) comprises the maintenance basal medium and supplement and allows for long-term maintenance of hPSC-derived cardiomyocytes for one month or longer. These cardiomyocytes can be used in various downstream applications and analyses.

Subtype
Specialized Media
 

Cell Type
Cardiomyocytes, PSC-Derived
 

Species
Human
 

Application
Cell Culture, Differentiation
 

Brand
STEMdiff
 

Area of Interest
Cell Line Development, Disease Modeling, Drug Discovery and Toxicity Testing, Stem Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- Simple monolayer protocol, compatible with STEMdiff™ cardiomyocyte workflow

- Establishment and efficient generation of up to 50 million atrial cardiomyocytes per kit

- Cardiomyocytes generated with this serum-free kit express high levels of atrial-specific genes and demonstrate dominant atrial-like phenotypes including faster beat rate and decreased field potential duration when compared to those generated using the STEMdiff™ Cardiomyocyte Differentiation Kit

- Maximum reproducibility with minimal variability across multiple hPSC lines

What's Included

- STEMdiff™ Cardiomyocyte Differentiation Basal Medium, 380 mL

- STEMdiff™ Atrial Cardiomyocyte Differentiation Supplement A (10X), 10 mL

- STEMdiff™ Atrial Cardiomyocyte Differentiation Supplement B (10X), 10 mL

- STEMdiff™ Atrial Cardiomyocyte Differentiation Supplement C (10X), 20 mL

- STEMdiff™ Cardiomyocyte Maintenance Basal Medium, 490 mL- STEMdiff™ Atrial Cardiomyocyte Maintenance Supplement (50X), 10 mL

Catalog #: 100-0215

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.